The animation character faces are examined to study their physiognomic appearance and personality. Faces of twelve animation characters were selected. Ninety-one students rated their faces on 20 scales concerning physiognomic appearance and 20 scales concerning personality. A five factor solution (full-faces/fragile/nose/eye/brow) in physiognomic appearance and a four-factor solution (affinity/vigor/negative/cautious) in personality were obtained via factor analysis. And these nine factors may discriminate among animation characters in several production companies via discriminant analysis.